Tris HCl 1.0M pH6.8 is a buffer useful for casting stacking gel.

Storage: 4 ° C

Bis-(2-Hydroxymethyl)imino-tris(hydroxymethyl) Methane
Ultra Pure Grade
MW 209.2 Purity: >99.0%
pKa ( 25 ° C): 6.50±0.2
Melting range: 104 ± 2 ° C

Storage: Room Temperature

Biotechnology Grade
MW 288.38
Purity: >99.0%
A260 (3%,water): <0.1 A280 (3%,water): <0.1
Chloride: <0.1% Phosphate: <0.005%
Pb: <0.005% Cu: <0.005%
DNase, RNase: Not detected

Storage: Room Temperature

Sodium Dodecyl Sulphate 10g/100ml, the ionic detergent which binds strongly to the proteins.

Storage: Room Temperature

This buffer is used as tank buffer for SDS-PAGE. The buffer at 1X concentration contains 0.025M Tris, 0.192M glycine and 0.1%SDSpH 8.3-8.6.

Storage: 4 ° C

This buffer is used as tank buffer for Native PAGE. The buffer at 1X concentration contains 0.025M Tris and 0.192M Glycine pH 8.3-8.6.

Storage: 4 ° C

This buffer is used as tank buffer for SDS-PAGE. The buffer at 1X concentration contains 0.1M Tris, 0.1M Tricine and 0.1% SDS pH 8.3-8.6.

Storage: 4 ° C

This buffer is used as Agarose Gel Electrophoresis buffer. The buffer at 1X concentration contains 0.02MTris, 0.1M Borate and 1mM EDTA pH7.6

Storage: 4 ° C

This buffer is used as electro-transfer buffer for protein blotting from SDS-PAGE. The buffer at 1x concentration contains 15mM Tris and 120mM Glycine, 0.1% SDS and 20% Methanol pH 8.6.

Storage: 4 ° C

Sample buffer at 5x concentration contains 4% SDS, 20% Glycerol, 10% b -mercaptoethanol, 0.004% bromophenol blue and 0.125M Tris-HCl, pH approximately 6.8. This is used for preparing sample for SDS-PAGE

Storage: 4 ° C

Sample buffer at 5X concentration contains 20% Glycerol, 0.004% bromophenol blue and 0.125M Tris-HCl pH approximately 6.8. This is used for preparing sample for Native PAGE.

Storage: 4 ° C

Content of one bottle of brilliant blue G stain is to be made to a final volume of 1litre with deionized H 2 O. The final solution will contain 0.1% (w/v) brilliant blue G, 25% methanol and7%Acetic acid. This is used for staining protein in PAGE and Agarose gels.

Storage: 4 ° C

Content of one bottle of brilliant blue R stain is to be diluted to one litre with deionized H 2 O. The final solution contains 0.25% (w/v) brilliant blue R, 40% methanol and 7% Acetic acid. This is suitable for staining protein in polyacrylamide and agarose gels.

Storage: Room Temperature

After dilution to 1X concentration the solution will contain 0.1% (w/v) amido black, 25% (w/v) isopropanol and 10% Acetic acid. This is suitable for staining proteins on nitrocellulose membranes after transfer from SDS-PAGE visualizes proteins in microgram range with a clear background.

Storage: Room Temperature

After dilution to 1X concentration the solution will contain 0.2% ponceau’s, 3%TCA. This is suitable for staining proteins on nitrocellulose membranes after transfer from SDS-PAGE. Can visualize proteins in microgram range with a clear background.

Storage: Room Temperature

Content of one bottle of destainer is to be diluted 4 times with deionized water. The final solution contains 15% methanol and 7.5% Acetic acid. This is suitable for destaining polyacrylamide and agarose gels.

Storage: Room Temperature