Principle
This kit is designed for fast isolation of genomic DNA from bacteria. The kit contains a spin column for binding up to 10µg of genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the MX-10 Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting etc.
Applications
- Genomic DNA purification from different sources
- PCR
- Southern Blotting
- RAPD, AFLP or RFLP
Features
- Preparation of high quality genomic DNA from different sources.
- Rapid and economical. Entire procedure takes about 15 minutes (Time for preparation of samples is not included).
- High yield and Reproducible
- No phenol / chloroform extraction , no ethanol precipitation
Procedure
Samples are lysed (see protocol), buffering conditions are adjusted, and lysate is loaded onto the spin column. DNA is selectively bound to the MX-10 column while impurities pass through. Remaining impurities are washed away. Pure genomic DNA is eluted in water or low-salt buffer, ready to use in a variety of downstream applications.
0.8% Agarose Gel. Genomic DNA preparations from bacterial samples using the Kit. The left lane is DNA Marker.| Kit Components | MX-0611-01 (50 Preps) | MX-0611-02 (100 Preps) |
| Digestion Solution | 20ml | 40ml |
| Wash Solution | 12ml | 24ml |
| Elution Buffer | 5ml | 10ml |
| Proteinase K | 2mg | 4mg |
| MX-10 Column | 50 | 100 |
| 2.0ml Collection Tube | 50 | 100 |
| Protocol | 1 | 1 |
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0611-01 | MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit (Bacteria) | 20 preps |
| MX-0611-02 | MEDOX-Easy Ultrapure Genomic DNA Spin Minipreps Kit (Bacteria) | 50 preps |
| MX-0611-03 | MEDOX-Easy® Ultrapure Genomic DNA Spin Minipreps Kit (Bacteria) | 100 preps |
Principle
This kit is designed as a quick one step method for DNA extraction from bacteria. This isolated DNA act as DNA template for PCR amplification. This yield enough to 50 PCR reactions set up.
Features & Application
- The rapid isolation of DNA from both gram-positive and gram-negative organisms.
- The isolated DNA is suitable for medical diagnosis of PCR amplifications.
- No binding resin-avoids shearing
- No RNase treatment required
- Does not involve organic solvent extractions. (ex. Phenol, chloroform)
Kit Components & Storage
DNA Extraction solution Kit Protocol
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0657-01 | MEDOX-Easy Bacterial Genomic DNA Extraction Kit (Quick One Step Method) | 100 extractions |
Principle
This MX-10 spin column mini-preps kit is designed for a fast isolation of genomic DNA from clinical samples such as fresh or frozen whole blood (with common anti-coagulants such as citrate, EDTA, and heparin), serum, bone, other body fluids and cell suspensions. 200µl-400µl per column of healthy whole blood sample is enough to obtain 6-12µg of pure genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the MX-10 Column. Purified blood genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting etc.
Applications
- Extraction of Genomic DNA from whole blood and other body fluids.
Features
- High quality of genomic DNA from blood and other clinical samples
- High yield and Reproducible
- No phenol / chloroform extraction , no ethanol precipitation
Storage
With the exception of the Proteinase K, the kit may be stored at room temperature. The proteinase K should be stored at -20ºC. The kit is stable for 12 months at room temperature. For longer storage, keep all contents cold.
0.8%Agarose Gel. GenomicDNApreparations from a single blood samples of whole human blood using the Kit. The left lane is DNA Marker.| Kit Components | MX-0607-01 (50 Preps) | MX-0607-02 (100 Preps) |
| TBP Buffer | 120ml | 2 x 120ml |
| TBM Buffer | 25ml | 50ml |
| TE (pH 8.0) | 15ml | 30ml |
| Proteinase K | 2mg | 4mg |
| Wash Solution | 12ml | 24ml |
| Elution Buffer | 5ml | 10ml |
| MX-10 Column | 50 | 100 |
| 2.0ml Collection Tube | 50 | 100 |
| Protocol | 1 | 1 |
Principle
This kit is used for rapid isolation and effectively reproducible, high yield og genomic DNA from blood sample
Features & Application
- The DNA extracted from 200 ul of blood sample.
- The isolated DNA is highly pure form, its suitable for PCR applications.
- No binding resin-avoids shearing
- No RNase treatment required
- Does not involve organic solvent extractions. (ex. Phenol, chloroform)
Kit Components & Storage
- Cell lysis buffer
- DNA Extraction solution
- Kit Protocol
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0658-01 | MEDOX-Easy Whole Blood DNA Extraction Kit | 50 extractions |
Principle
This kit is designed for fast isolation of genomic DNA from animal tissues. The kit contains a spin column for binding up to 10µg of genomic DNA. Nucleotides, proteins, salts and other impurities do not bind to the MX-10 Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting etc.
Applications
- Genomic DNA purification from different sources
- PCR
- Southern Blotting
- RAPD, AFLP or RFLP
Features
- Preparation of high quality genomic DNA from variable sources.
- Rapid and economical. Entire procedure takes about 15 minutes (Time for preparation of samples is not included).
- High yield and Reproducible
- No phenol / chloroform extraction , no ethanol precipitation
Procedure
Samples are lysed (see protocol), buffering conditions are adjusted, and lysate is loaded onto the spin column. DNA is selectively bound to the MX-10 column while impurities pass through. Remaining impurities are washed away. Pure genomic DNA is eluted in water or low-salt buffer, ready to use in a variety of downstream applications.
Gel electrophoresis of a animal genomic DNA sample purified by using EZ-10 Spin Column Genomic DNA Minipreps Kit. The lane at the left end is DNA marker.
| Kit Components | MX-0608-01 (20 Preps) | MX-0608-02 (50 Preps) |
| ACL Solution | 20ml | 40ml |
| AB Solution | 20ml | 40ml |
| PBS Solution | 20ml | 40ml |
| Proteinase K | 20mg | 40mg |
| Wash Solution | 12ml | 24ml |
| Elution Buffer | 5ml | 10ml |
| MX-10 Column | 50 | 100 |
| 2.0ml Collection Tube | 50 | 100 |
| Protocol | 1 | 1 |
Principle
This kit is designed for fast isolation of genomic DNA from plant tissues. The kit contains a spin column for binding up to 10µg of genomic DNA. Nucleotides,proteins, salts and other impurities do not bind to the MX-10 Column. Purified genomic DNA can be applied in most molecular biology experiments including restriction digestion, PCR, Southern-blotting etc.
Applications
- Genomic DNA purification from different sources
- PCR
- Southern Blotting
- RAPD, AFLP or RFLP
Features
- Preparation of high quality genomic DNA from variable sources.
- Rapid and economical. Entire procedure takes about 15 minutes (Time for preparation of samples is not included).
- High yield and Reproducible
- No phenol / chloroform extraction, no ethanol precipitation
Procedure
Samples are lysed (see protocol), buffering conditions are adjusted, and lysate is loaded onto the spin column. DNA is selectively bound to the MX-10 column while impurities pass through. Remaining impurities are washed away. Pure genomic DNA is eluted in water or low-salt buffer, ready to use in a variety of downstream applications.
Gel electrophoresis of a plant genomic DNA sample purified by using MX-10 Spin Column Genomic DNA Minipreps Kit. The lane at the left end is DNA marker.
| Kit Components | MX-0623 (50 Preps) | MX-0624 (100 Preps) |
| PCL Solution | 15ml | 30ml |
| PP Solution | 2ml | 4ml |
| PB Solution | 20ml | 40ml |
| Wash Solution | 12ml | 24ml |
| Elution Buffer | 5ml | 10ml |
| MX-10 Column | 50 | 100 |
| 2.0ml Collection Tube | 50 | 100 |
| Protocol | 1 | 1 |
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0612-01 | MEDOX-Easy Genomic Plant DNA Extraction Kit | 10 preps |
| MX-0612-02 | MEDOX-Easy Genomic Plant DNA Extraction Kit | 50 preps |
The Plant material is suspended in CTAB genomic DNA extraction buffer. Cells are then lysed by osmotic shock and salt precipitate the cell debris. Proteins are then precipitated using phenol chloroform and followed by ethanol precipitation of DNA.
Features & Application
- Mainly used to prepare pure high molecular weight DNA for gene manipulation.
- Used to construct genomic DNA libraries and to probe the plant genome for the presence of DNA markers such as RAPD and RFLP.
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0661-01 | MEDOX-Easy Genomic Plant DNA Extraction Kit (CTAB Method) | 10 preps |
| MX-0661-02 | MEDOX-Easy Genomic Plant DNA Extraction Kit (CTAB Method) | 50 preps |
Principle
This kit is designed as a quick one step method for DNA extraction from bacteria. This isolated DNA act as DNA template for PCR amplification. This yield enough to 50 PCR reactions set up.
Features & Application
- Rapid isolation of DNA from monocot and dicot leaves.
- The highly pure isolated DNA is suitable for PCR application.
- No binding resin – avoids shearing
- No RNase treatment required
- Does not involve organic solvent extractions (ex. Phenol, chloroform)
Kit Components & Storage
- Cell lysis buffer
- DNA Extraction solution
- Kit Protocol
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0612-05 | MEDOX-Easy Plant DNA Extraction for PCR Amplification | 100 extractions |
(For purification of PCR products and DNA cleanup of variable enzymatic reactions.)
| KitComponents |
MX-0601-01
(50 Preps) |
MX-0601-02
(100 Preps) |
| Binding Solution I |
20ml
|
40ml
|
| Wash Solution |
12ml |
24ml |
| Elution Buffer |
5ml |
10ml |
| EZ-10 Column |
50 |
100 |
| 2.0ml Collection Tube |
50 |
100 |
| Kit Protocol |
1 |
1 |
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0601-01 | MEDOX-Easy Spin Column PCR Products Purification Kit | 50 preps |
| MX-0601-02 | MEDOX-Easy Spin Column PCR Products Purification Kit | 100 preps |
| Kit Components |
MX-0609
(50 Reactions) |
MX-0610
(100 Reactions) |
| MX-10 Column |
50 |
100 |
| Fast DNA ligase 2.0ml Collection tube |
50 |
100 |
| Binding Solution II |
30ml |
60ml |
| Wash Solution |
12ml |
24ml |
| Elution Buffer |
5ml |
10ml |
| Protocol |
1 |
1 |
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0605-01 | MEDOX-Easy Spin Column DNA Gel Etraction Kit | 50 preps |
| MX-0605-02 | MEDOX-Easy Spin Column DNA Gel Etraction Kit | 100 preps |
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0623-01 | Single Step Ultra-Competent Cell Preps Kit | 25ml |
| MX-0623-02 | Single Step Ultra-Competent Cell Preps Kit | 50ml |
Principle
This kit is designed for fast isolation of total RNA from bacteria, yeast, fungi, plant and animal tissues. This kit can also be used to clean up RNA, which are isolated by different methods or after enzymatic reactions. The kit contains a membrane embedded in a spin column for binding upto 10µg of RNA. Nucleotides, proteins, salts and other impurities do not bind to the MX-10 Column.
Applications
- Preparation of Total RNA from variable sources.
- RT-PCR
- Differential display
- cDNAsynthesis
- PolyA + RNA selection
- RNase/S1 nuclease protection
Features
- Preparation of High quality total RNA from animal cells or other sources.
- Rapid and Economical. Entire procedure takes about 20 minutes (Timing for preparation of samples is not included).
- High yield and Reproducible
- No phenol / chloroform extraction , no ethanol precipitation
Procedure
The detailed procedures for Isolation of Total RNA from Animal Cells, Bacteria, Tissue, Plant Cells and Filamentous Fungi and for RNA clean up are supplied with kits.
Procedure
The detailed procedures for Isolation of Total RNA from Animal Cells, Bacteria, Tissue, Plant Cells and Filamentous Fungi and for RNA clean up are supplied with kits.
Gel electrophoresis of the total RNA from the leaf purified by using Spin Column
Gel electrophoresis of the total RNA from the bacteria purified by using Spin Column
* Solution A may form a precipitate upon storage. If necessary, re-dissolve it by warming it at 37 ° CSolution B should be kept at 2-8 ° C after receiving and usage.
| Kit Components | MX-0617-01 (5 Preps) |
MX-0617-02
(20 Preps) |
MX-0617-03 (100 Preps) |
| RLT Solution A | 3.5ml |
14ml |
70ml |
| RW Solution | 3 ml |
12ml |
60ml |
| RPE Solution B | 1.25ml |
5ml |
22ml |
| DEPC-Water | 0.25ml |
1ml |
5ml |
| EZ-10 Column | 5 |
20 |
100 |
| 2.0ml Collection Tube | 5 |
20 |
100 |
| Protocol | 1 |
1 |
1 |
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0617-01 | MEDOX-Easy Spin Column Total RNA Minipreps Super Kit | 5 preps |
| MX-0617-02 | MEDOX-Easy Spin Column Total RNA Minipreps Super Kit | 20 preps |
| MX-0617-03 | MEDOX-Easy Spin Column Total RNA Minipreps Super Kit | 100 preps |
Principle
This kit provides a convenient and stright forward method for purification of mRNA from cells or tissues. The kit contains a guanidine-thiocyanate based buffer for efficient cell and tissue lysis, protein denaturation and RNase inactivation to keep mRNA intact. Homogenized cells or tissue lysates containing total RNA are incubated with Oligo-dT. mRNA is selectively absorbed on the Oligo-dT cellulose and other impurities are washed away. The mixture containing mRNA. Oligo-dT Cellulose is placed on the MX-10 column. While mRNA is released by R-Elution Buffer in small volume. This kit is used for 100 mg of tissue sample or 10 6 cells.
Applications
- RNA blotting
- In vitro translation
- Differential display
- CDNA synthesis or library construction
- RT-PCR
- Microinjection
Features
- Fast and simple
- Reproducible
Storage
The kit is stable for 12 months at room temperature.
| Kit Components | MX-0649-01 (10 Preps) | MX-0649-02 (20 Pre ps) |
| Digestion Solution | 10 ml | 20ml |
| 2M NaAC pH4 | 1 ml | 2ml |
| RNA Binding Buffer | 15ml | 30ml |
| R-Elution Buffer | 15ml | 30ml |
| MX-10 Column | 10 | 20 |
| Oligo-dt cellulose | 10×0.5mg | 20×0.5mg |
| Protocol | 1 | 1 |
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0649-01 | MEDOX-Easy mRNA Purification Kit | 10 Preps |
| MX-0649-02 | MEDOX-Easy mRNA Purification Kit | 20 Preps |
Description
- Total RNA Extraction Reagent (TRIZOL) is a ready- to-use reagent for the isolation of total RNA from cells and tissues.
- This technique performs well with small quantities of tissue (50~100mg) and cells (5 x 10 6 ), and large quantities of tissue (>1g) and cells (>10 7 ), of human, animal, plant, or bacterial origin.
- Total RNA Extraction Reagent (TRIZOL) facilitates isolation of a variety of RNA species of large or small molecular size.
Storage Conditions: 2~8°C
Total RNA Extraction Reagent (TRIZOL) has demonstrated stability of 12 months when stored at 2~8°C for optimal performance.
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0619-01 | MEDOX-Easy Total RNA Extraction Reagent (Tri-Reagent) | 25ml |
| MX-0619-02 | MEDOX-Easy Total RNA Extraction Reagent (Tri-Reagent) | 100ml |
It is a Di-sodium salt of Ethylene Di-amine Tetracetate, prepared in Milli-Q water with pH adjusted to 8 with NaOH. It is used in preparation of Nucleic acids, Proteins, etc.,
Storage: Room Temperature
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0634-01 | 0.5M EDTA (pH 8) | 50ml |
reated with DEPC, filtered and autoclaved H 2 O : 18.02 Residue after evaporation : <3 ppm
Storage: Room Temperature
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0630-03 | Water (RNase-Free) | 100ml |
| MX-0630-04 | Water (RNase-Free) | 5x100ml |
Biotechnology Grade
MW : 118.16
Purity : >99.0%
Melting range : 118-121 ° C
Solubility (70%,H 2 O, 25 ° C) : Clear
A280 (70%,H 2 O, 25 ° C) : <0.8
DNase, RNase : None detected
Storage: Room Temperature
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0631-01 | Guanidine Thiocyanate | 50g |
| MX-0631-02 | Guanidine Thiocyanate | 100g |
Human Placental Source Biotechnology Grade
Purity : >95%
DNase (exonuclease, endonuclease) : None detected
Concentration: 10 units/ml
Storage : -20°C
Storage buffer :20mM HEPES-KOH, pH 7.6, 50mM KCl, 8mM DTT and 50% glycerol| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0632-01 & MX-0362-02 | RNase Inhibitor | 1000U |
Bovine Pancreas Ultra pure (Lyophilized powder)
Activity : Approx. 2,000 U/mg
RNase activity : None detected
Storage : -20°C
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0711-01 | DNase I (RNase Free) | 1000U |
| MX-0711-02 | DNase I (RNase Free) | 2000U |
Phenol, redistilled and saturated with DEPC water and Tris-HCl seperately
Storage: 4 ° C
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0637-01 | Phenol – Water Saturated | 50ml |
| MX-0637-02 | Phenol – Water Saturated | 300ml |
| MX-0638-01 | Phenol – Tris Saturated | 50ml |
| MX-0638-02 | Phenol – Tris Saturated | 300ml |
It consists of 0.025% Bromophenol Blue and 30% glycerol. Used
as a tracking dye for agarose gel electrophoresis at the
concentration of 1X.C
Storage: 4°C
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0902-01 | 6X Gel Loading Dye | 6 x 1ml |
High Purity Grade
Strong inhibitor of RNase activity.
MW : 162.14
Purity : >97.0%
Storage: 4°C
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0228-01 | DEPC | 5 ml |
| MX-0228-02 | DEPC | 25 ml |
Activity (Kunits) : >70 U/mg.
Anessentially Protease-free, Chromatographically prepares
Lyophilized powder from Bovine Pancreas.
Storage : -20°C
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0710-01 | Rnase A (Dnase Free) | 1ml |
| MX-0710-02 | Rnase A (Dnase Free) | 10mg |
| MX-0710-03 | Rnase A (Dnase Free) | 50mg |
| MX-0710-04 | Rnase A (Dnase Free) | 100mg |
| MX-0710-05 | Rnase A (Dnase Free) | 500mg |
Trititrachium album
Biotechnology Grade
Activity : >30.0 U/mg
DNase, RNase activity : None detected
Storage : -20°C
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0709-01 | Proteinase K | 25mg |
| MX-0709-02 | Proteinase K | 100mg |
| MX-0709-03 | Proteinase K | 500mg |
| MX-0709-04 | Proteinase K | 1g |
5M Sodium acetate of pH 5.2 adjusted with 0.1N Glacial Acetic acid.
Storage : Room Temperature
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-1063-01 | Sodium Acetate 5M | 100ml |
3M Sodium acetate of pH 5.2 adjusted with Glacial Acetic acid. This is used in DNA preparation.
Storage: Room Temperature
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0635-01 | Sodium Acetate 3M | 100ml |
3M Sodium acetate of pH 5.2 adjusted with Glacial Acetic acid. This is used in DNA preparation.
Storage: Room Temperature
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0635-01 | Sodium Acetate 3M | 100ml |
10X MOPS Buffer provide in a powder form contains all the components to make 250ml of 10X concentration of MOPS Buffer (Stock).
MOPS Buffer used as a running buffer in agarose gel electrophoresis for northern blotting.
Storage: 4 ° C in dark bottle
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0651-01 | 10X MOPS Buffer | 250 ml |
10mM Tris acid 1mM EDTA Buffer with pH 8.
Used for rehydration of DNA extraction.
Storage: Room Temperature
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0636-01 | 10X TE Buffer | 100ml |
It consists of bromophenol blue and xylene cyanol. Used as a RNA tracking dye in agarose gel electrophoresis.
Storage: 4 ° C
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0652-01 | 5X RNA Tracking Dye | 2 x 0.5 ml |
It consists of 0.025% Bromophenol Blue and 30% glycerol. Used
as a tracking dye for agarose gel electrophoresis at the
concentration of 1X.
Storage: 4 ° C
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0902-01 | 6X Gel Loading Dye | 6 x 1ml |
It consists of 0.025% Orange G and 30% glycerol. Used as a
tracking dye for agarose gel electrophoresis.
Storage: 4 ° C
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-0903-01 | 6X Orange Gel Loading Dye | 6 x 1ml |
High Purity Grade
Strong inhibitor of RNase activity.
MW : 162.14
Purity : >97.0%
Storage: 4 ° C
| Ordering Information | ||
| Cat.No | Product | Units |
| MX-1064-01 | Phenol:Chloroform:Isoamyl alcohol | 50 ml |