Conjugation Grade

Specific activity : >250 Purp. U/mg

RZ : >3.0

Solubility (1%, water) : Clear

Storage : -20°C

Supplied with 10X Reaction Buffer(Ligation Buffer) T4 DNA ligase catalyzes a joining reaction between DNA fragment, involving the 3′-OH and the 5′-phosphate termini. The reaction requires ATP and Mg 2+ . The enzyme also catalyzes the exchange of free pyrophosphate into an activated carbon absorbable compound.

Concentration : 100U/µl

Unit Definition : 1 unit is defined as the amount of enzyme required to 50% ligation of Hind III-fragments of lambda DNA in 30 minutes at 16 ° C in 20µl of reactions mixture with DNA concentration about 300µg/ml

Reaction Buffer : 50mM Tris-HCl (pH 7.8), 10mM MgCl 2 , 10mM DTT, 1mM ATP and 25µg/ml BSA

Storage Buffer : 20mM Tris-HCl (pH 7.5), 50mM KCl, 10mM 2-Mercaptoethanol, 1mM EDTA and 50% glycerol

Storage : -20°C

Trititrachium album

Biotechnology Grade

Activity : >30.0U/ mg

DNase, RNase activity : None detected

Storage : -20°C

DNase free, Pancreatic, High Purity Grade

Activity : >60U/mg

Purity : >99.0%

DNase : None detected

Storage : -20°C

Bovine Pancreas Lyophilized powder

Ultra pure

Activity : Approx. 2,000 U/mg/p>

RNase activity : None detected

Storage : -20°C

Biotechnology Grade

Activity (25°C) : >20,000U/mg

Albumin : None detected

Salmonella : None detected

RNase, DNase, Protease : None detected

Storage : -20°C