Nucleic Acid Electrophoresis

Products

Nucleic Acid Electrophosis reagets

Recommended for Nucleic acid fragments with sizes >1000bp. This agarose is also suitable for blotting assays. DNase / RNase activity not detected.

Storage: Room Temperature

Ordering Information
Cat.No Product Units
MX-0906-01 Agarose Low EEO 50g
MX-0906-02 Agarose Low EEO 100g
MX-0906-03 Agarose Low EEO 500g
MX-0906-04 Agarose Low EEO 1Kg

Recommended for serum protein electrophoresis and immuno electrophoresis.
DNase / RNase activity not detected.

Storage: Room Temperature

Ordering Information
Cat.NoProductUnits
MX-0907-01Agarose Medium EEO50g
MX-0907-02Agarose Medium EEO100g
MX-0907-03Agarose Medium EEO1Kg

Recommended for immuno electrophoresis. DNase / RNase activity not detected.

Storage: Room Temperature

Ordering Information
Cat.NoProductUnits
MX-0908-01Agarose High EEO50g
MX-0908-02Agarose High EEO100g
MX-0908-03Agarose High EEO1Kg

Recommended for 1000bp fragment separation in analytical electrophoresis. The low melting temperature of this agarose (65 ° C) allows the gel to be melted without damaging the DNA, so the fragments can be recovered. Dnase / Rnase activity not detected.

Storage: Room Temperature

Ordering Information
Cat.NoProductUnits
MX-0905-01Agarose Low melting point10g
MX-0905-02Agarose Low melting point100g

Prehybridization buffer is 2X in concentration, the buffer is prepared in a nuclease free water, it contains SSC buffer, sonicated Salmon sperm DNA, Denharts solution.

Application:

In pre-hybridization step, filter (membrane) is incubated in a prehybridization buffer, which is designed to precoat or block the membrane, then probe bind non-specifically to the template DNA. In hybridization pre-hybridization step is important, fail to do this step leads to high background.

Storage: Prehybridization buffer is supplied in frozen condition

Ordering Information
Cat.No Product Units
MX-0653-01 Prehybridization Buffer 2X 50 ml

Hybridization buffer is 2X in concentration, the buffer is prepared in a nuclease free water, it contains SSC buffer, Denharts solution.

Application:

In Hybridization step, filter (membrane) is incubated in a hybridization buffer, with probe.

Storage: Hybridization buffer is supplied in frozen condition.

Ordering Information
Cat.NoProductUnits
MX-0654-01Hybridization Buffer 2X50 ml

A single-strength (1X) solution contains 0.089M Trisbase, 0.089M borate and 0.002M EDTA.

Application:

10X TBE used as running buffer for agarose gel electrophoresis at the working concentration of either 0.5X or 1X

Storage: Room Temperature.

Ordering Information
Cat.NoProductUnits
MX-0900-0110X TBE Buffer200ml

A single strength (1X) solution contains 0.04M Tris base, 0.04M acetate, and 0.001M EDTA/p>

Application:

50X TAE used as running buffer for agarose gel electrophoresis at the working concentration of 1X

Storage: Room Temperature.

Ordering Information
Cat.No Product Units
MX-0900-01 10X TBE Buffer 200ml

It consists of 0.025% Bromophenol Blue and 40% Sucrose, used as a tracking dye for agarose gel electrophoresis at the concentration of 1X.

Storage: 4 ° C

Ordering Information
Cat.NoProductUnits
MX-0902-016X Gel Loading Dye6 x 1ml

Biotechnology Grade

MW 394.32

Purity: >98.0%

Moisture: <8.0%

Ammonium Bromide: <0.1%

Storage: Room Temperature

Ordering Information
Cat.NoProductUnits
MX-0904-01Ethidium Bromide (Powder)10mg
MX-0904-02Ethidium Bromide (Powder)50mg
MX-0904-03Ethidium Bromide (Powder)100mg
MX-0904-04Ethidium Bromide (Powder)1g

A readily prepared solution and can be used as such. It acts as an intercalating agent to nucleic acids, which fluorescence under UV (to stain nucleic acid).

Storage: Room Temperature

Ordering Information
Cat.NoProductUnits
MX-0904-03Ethidium Bromide (Solution)1ml

It is used in gel and running buffer to visualize DNA.
The migration of the DNA can be monitored, as bands are visible in ambient light.

Ordering Information
Cat.NoProductUnits
MX-0971-0110X Gel Staining Solution (for Nucleic Acid)100 ml

it consists of 0.025% Orange G and 30% Glycerol. Used as a tracking dye for agarose gel electrophoresis/p>

Storage : 4°C

Ordering Information
Cat.NoProductUnits
MX-0903-016X Orange Gel Loading Dye6X1ml

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