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MEDOX-BIOTM Dot ELISA Teaching Kit
MEDOX-BIOTM Antigen Captigen Capture ELISA Teaching Kit
MEDOX-BIOTM Antibody Capture ELISA Teaching Kit
MEDOX-BIOTM Sandwich ELISA Teaching Kit
MEDOX-BIOTM Competitive ELISA Teaching Kit
 
MEDOX-BIOTM Dot ELISA Teaching Kit
 

The Dot ELISA teaching kit is based on the principle of ELISA wherein the antigen is coated on the Nitrocellulose strips (instead of microtitre plate) and bind with specific antibody and this complex is conjugated with enzyme and react with substrate to develop a clear dot at the final step.

Features & Application

* With this kit the students can learn to coat the antigen in Nitrocellulose strips and can learn the principle of ELISA without ELISA Reader.

Kit Components & Storage
(Enough to perform 15 Experiments)

Antigen (-20°C)

Antibody (-20°C)

Blocker (4°C)

1X PBS (RT)

Washing buffer (RT)

Conjugate (4°C)

Substrate (-20°C)

H2O2 (4°C)

Nitrocellulose Strips (RT)

Normal Saline (RT)

Test Antigen (-20°C)

Kit Protocol




Ordering Information

Cat.No

Product

Units

MX-1507-01

MEDOX-BIOTM Dot ELISA Teaching Kit

15 Exps.

 

 

MEDOX-BIOTM Antigen Captigen Capture ELISA Teaching Kit
 

The antibody captures the sample antigen and this antigen-antibody complex bind to an enzyme labelled conjugate. Then, this complex is allowed to react with substrate for colour formation.

Features & Application

* To teach students about the quantitative detection of antigen by ELISA method.

Kit Components & Storage
(Enough to perform 4 Experiments)

Antigen (-20°C)

Antibody (-20°C)

Blocker (4°C)

Sample I & II (-20°C)

Coating Buffer (RT)

Conjugate (4°C)

Substrate (-20°C)

H2O2 (4°C)

Normal Saline (RT)

Washing buffer (RT)

ELISA module strips

1X PBS (RT)

Substrate Buffer (4°C)

Stopping solution (RT)

Kit Protocol

 


Ordering Information

Cat.No

Product

Units

MX-1508-01

MEDOX-BIOTM Antigen Capture ELISA Teaching Kit

4 Exps

 

 

MEDOX-BIOTM Antibody Capture ELISA Teaching Kit
 

This kit is based on the principles of ELISA. A constant dilution of antigen is coated on a solid phase. To titrate antibody at different dilutions added. Antibody will capture and bind with the specific antigen coated in the well. Bound antibody is detected by addition of enzyme labeled conjugate specific for the captured antibody. This complex is finally assayed by adding the substrate that induces the colour reaction.

Features & Application
* Helps students to learn about the quantitative detection of antibody from a test sample against the fixed amount of antigen.
* Used for diagnosis & research purposes

Kit Components & Storage
(Enough to perform 4 Experiments)

Antigen(-20°C)

Antibody (-20°C)

Blocker (4°C)

Sample I & II (-20°C)

Coating Buffer (4°C)

Conjugate (4°C)

Substrate (-20°C)

Substrate Buffer (4°C)

H2O2 (4°C)

Washing buffer (RT)

Normal Saline (RT)

1X PBS (RT)

ELISA module strips (RT)

Kit Protocol



Ordering Information

Cat.No

Product

Units

MX-1509-01

MEDOX-BIOTM Antibody Capture ELISA Teaching Kit

4 Exps.

 

 

MEDOX-BIOTM Sandwich ELISA Teaching Kit
 

A constant dilution of antibody is coated on a solid phase. Specific antigen will bind with the specific antibody coated in the well. This complex is to bind with antibody enzyme labeled conjugate and finally the captured antigen is sandwiched in between two antibodies. Hence this principle is called sandwich ELISA. Then by adding the substrate the colour reaction will take place.

Features & Application
* Simple method to teach the techniques of Sandwich ELISA in a very easy and understandable manner.

Kit Components & Storage
(Enough to perform 4 Experiments)

Antigen (-20° C) Antibody (-20°C)
Blocker (4°C) Sample I & II (-20°C)
Coating Buffer (4°C) Conjugate (4°C)
Substrate (-20°C) Substrate Buffer (4°C)
H2O2 (4°C) Washing buffer (RT)
Normal Saline (RT) ELISA module strips (RT)
1X PBS (RT) Kit Protocol

Ordering Information

Cat.No

Product

Units

MX-1510-01

MEDOX-BIOTM Sandwich ELISA Teaching Kit

4 Exps

 

 

MEDOX-BIOTM Competitive ELISA Teaching Kit
 

This kit is based on the principle of Competitive ELISA. In this method, two reactants are trying to bind to a third reactant. Constant amount of antigen is coated on a solid phase. Then by simultaneous addition of antibody and competitor antigen, the solid phase antigen is challenged by the addition of diluted ranges of antigen in the liquid phase. The antibody labeled enzyme conjugate binds with antigen antibody complex and colour is formed after the addition of substrate. Competition is reflected by a decreased colour formation.

Features & Application
* Easy to calculate the degree of inhibition.
* To teach the students for measuring the inhibition of competitor antigen of an unknown concentration.

Kit Components & Storage
(Enough to perform 4 Experiments)

Antigen (-20°C) Antibody (-20°C)
Blocker (4°C) Sample I & II (-20°C)
Coating Buffer (4°C) Conjugate (4°C)
Substrate (-20°C) Substrate Buffer (4°C)
H2O2 (4°C) Washing buffer (RT)
Normal Saline (RT) ELISA module strips (RT)
1X PBS (RT) Kit Protocol

Ordering Information

Cat.No

Product

Units

MX-1515-01

MEDOX-BIOTM Competitive ELISA Teaching Kit

4 Exps.

 

 

   
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