Note: This kit is used for preparation of up to 10µg of pure plasmid DNA. If large scale of preparation is needed, MX-200 column and MX-500 column are recommended.

• Plasmid DNA purification.
• Recovery of DNA fragments from reaction solutions. Removal of nucleotides and oligos (<40 - mer) & plasmid DNA clean up.

• Simple and fast. The entire procedure takes about 10-15 minutes.
• Preparation of high quality plasmid DNA from bacterial culture. Purified DNA can be used in any downstream applications such as sequencing, transformation, restriction enzymatic digestion and ligation.
• High yield (>80%) and reproducible.
• No phenol / chloroform extraction or ethanol precipitation.

All components in the kit are tested in purification of 10µg pUC 18 DNA from overnight culture.

Procedure

Bacterial cultures are first lysed, the lysates are cleared by routine method. Plasmid DNA from the cleared lysates is selectively bound to the silica based membrane in the MX-10 column and impurities are washed away. Pure plasmid DNA is eluted in low-salt buffer or water.

* Solution II may form a precipitate upon storage. If necessary, dissolve the precipitate by warming at 37 ° C.
** Before use for the first time, add four times of 96%-100% of ethanol to Wash Solution (v/v).