Recommended for Nucleic acid fragments with sizes >1000bp. This agarose is also suitable for blotting assays. DNase / RNase activity not detected.

Storage: Room Temperature

Recommended for serum protein electrophoresis and immuno electrophoresis.
DNase / RNase activity not detected.

Storage: Room Temperature

Recommended for immuno electrophoresis. DNase / RNase activity not detected.

Storage: Room Temperature

Recommended for 1000bp fragment separation in analytical electrophoresis. The low melting temperature of this agarose (65 ° C) allows the gel to be melted without damaging the DNA, so the fragments can be recovered. Dnase / Rnase activity not detected.

Storage: Room Temperature

Prehybridization buffer is 2X in concentration, the buffer is prepared in a nuclease free water, it contains SSC buffer, sonicated Salmon sperm DNA, Denharts solution.

Application:

In pre-hybridization step, filter (membrane) is incubated in a prehybridization buffer, which is designed to precoat or block the membrane, then probe bind non-specifically to the template DNA. In hybridization pre-hybridization step is important, fail to do this step leads to high background.

Storage : Prehybridization buffer is supplied in frozen condition

Hybridization buffer is 2X in concentration, the buffer is prepared in a nuclease free water, it contains SSC buffer, Denharts solution.

Application

In Hybridization step, filter (membrane) is incubated in a hybridization buffer, with probe.

Storage : Hybridization buffer is supplied in frozen condition.

The pre-hybridization and hybridization solution contains SSC based buffer and denatured fragmented Salmon sperm DNA for blocking membrane. The pre-hybridization and hybridization are important for maximum level of radio-labelled probe hybridization. For nitrocellulose based membrane pre-hybridization solution can also be used as hybridization solution.

A single-strength (1X) solution contains 0.089M Trisbase, 0.089M borate and 0.002M EDTA.

10X TBE used as running buffer for agarose gel electrophoresis at the working concentration of either 0.5X or 1X

Storage: Room Temperature

A single strength (1X) solution contains 0.04M Tris base, 0.04M acetate, and 0.001M EDTA

50X TAE used as running buffer for agarose gel electrophoresis at the working concentration of 1X

Storage: Room Temperature

It consists of 0.025% Bromophenol Blue and 40% Sucrose, used as a tracking dye for agarose gel electrophoresis at the concentration of 1X.

Storage: 4 ° C

Biotechnology Grade
MW 394.32
Purity: >98.0%
Moisture: <8.0%
Ammonium Bromide: <0.1%
Storage: Room Temperature

A readily prepared solution and can be used as such. It acts as an intercalating agent to nucleic acids, which fluorescence under UV (to stain nucleic acid).

Storage: Room Temperature

It is used in gel and running buffer to visualize DNA.
The migration of the DNA can be monitored, as bands are visible in ambient light.