Genomic DNA from different sources are prepared through Spin columns based methods. These purified high molecular weight Genomic DNA can be used in most molecular biology experiments including Restriction digestion, PCR, Southern blotting etc.

Storage Buffer : 10mM Tris-HCl(pH 8.0) and 1mM EDTA
Storage : -20°C

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For directly cloning PCR products with an overhang dA residue at 3' ends produced by Taq DNA polymerase, or other polymerases. This vector can also be used for cloning the blunt-ended DNA fragments which are needed to be added with an A-residue by using A-Tailing Kit (MX-0641) before cloning.

Storage : -20°C

Note: DNA ligase is not included in this kit. For purchasing T4 DNA ligase see Modifying Enzymes section.

Lambda DNA is used as one of the substrates in restriction enzymes research and for testing of restriction enzymes activity. Phage is isolated from heat inducible lysogen E.coli W3350 (cI857 Sam7). Lambda DNA is isolated from purified phage by phenol extraction and dialyzed against 10mM Tris-HCl (pH 7.6) and 1mM EDTA. It contains 48502 base pairs

Concentration : 500µg/ml
Storage Buffer : 10mM Tris-HCl(pH7.8) and 1mM EDTA.
Storage : -20°C

Lambda DNA is used as one of the substrates in restriction enzymes research and for testing of restriction endonuclease's sensitivity to Dam or Dcm methylation activity. Phage is isolated from heat inducible lysogen E.coli GM2163 (cI857 Sam7). Lambda DNA is isolated from purified phage by phenol extraction and dialyzed against10mMTris-HCl (pH 7.8) and 1mM EDTA. It contains 48502 base pairs.

Concentration : 500mg/ml
Storage Buffer : 10mM Tris-HCl(pH7.8)and1mM EDTA
Storage : -20°C

pUC18 DNA is isolated from E.coli strain DH5 a . Purified by ultra centrifugation through a cesium chloride gradient in the presence of ethidium bromide. The molecule is double-stranded, 2686 base pairs in length.

Concentration : 0.3-0.5mg/ml
Storage Buffer : Tris-HCl (pH7.8) and 1mM EDTA.
Storage : -20°C

pUC19 DNA is isolated from E.coli strain DH5 a . Purified by ultra centrifugation through a cesium chloride gradient in the presence of ethidium bromide. The molecule is double- stranded, 2686 base pairs in length.

Concentration : 200µg/ml
Storage Buffer : 10mM Tris-HCl (pH7.8) and 1mM EDTA.
Storage : -20°C

pUC57 DNA is isolated from E.coli strain DH5 a . Purified by ultra centrifugation through a cesium chloride gradient in the presence of ethidium bromide. The molecule is double- stranded, 2710 base pairs in length.

Concentration : 0.3-0.5mg/ml
Storage Buffer : 10mM Tris-HCl (pH7.8) and 1mM EDTA
Storage : -20°C

pBR322 DNA is isolated from E.coli (dam+, dcm+) and purified by ultra centrifugation. The molecule is a double-stranded circle, with 4361 base pairs in length.

Concentration : 200µg/ml
Storage Buffer : 10mM Tris-HCl(pH7.8) and 1mM EDTA
Storage : -20°C

Purified from bacteriophageT7. Homogenous using agarose gel electrophoresis.

Concentration : 500µg/ml
Storage Buffer : 10mM Tris-HCl (pH7.8) and 1mM EDTA.
Storage : -20°C